All Publications

2025

Thomas M. Lister, George W. Roberts, Euan J. Hossack, Fei Zhao, Ashleigh J. Burke, Linus O. Johannissen, Florence J. Hardy, Alexander A. V. Millman, David Leys, Igor Larrosa and Anthony P. Green, Engineered enzymes for enantioselective nucleophilic aromatic substitutions. Nature 639, 375-381 (2025). Read here.

Kseniia Korchagina, Sree Ganesh Balasubramani, Jordan Berreur, Emilie F. Gerard, Linus O. Johannissen, Anthony P. Green, Sam Hay, Steven D. Schwartz, Directed Evolution’s Selective Use of Quantum Tunneling in Designed Enzymes - A Combined Theoretical and Experimental Study. The Journal of Physical Chemistry B 129, 1555-1562 (2025). Read here.

2024

Florence J. Hardy, Matthew G. Quesne, Emilie F. Gérard, Jingming Zhao, Mary Ortmayer, Christopher J. Taylor, Hafiz S. Ali, Jeffrey W. Slater, Colin W. Levy, Derren J. Heyes, J. Martin Bollinger, Jr., Sam P. de Visser and Anthony P. Green* Probing Ferryl Reactivity in a Nonheme Iron Oxygenase Using an Expanded Genetic Code. ACS Catal, 11584-11590, (2024) Read here.

Zachary Birch-Price, Florence J. Hardy, Thomas M. Lister, Anna R. Kohn and Anthony P. Green* Noncanonical Amino Acids in Biocatalysis. Chemical Reviews, (2024) Read here.

Amy E. Hutton,   Jake Foster,   James E. J. Sanders, Christopher J. Taylor, Stefan A Hoffmann, Yizhi Cai, Sarah L. Lovelock*  and  Anthony P. Green*, An Efficient Pyrrolysyl-tRNA synthetase for Economical Production of MeHis-containing Enzymes. Faraday Discuss., 252, 295-305, (2024) Read here.

Amy E. Hutton, Jake Foster, Rebecca Crawshaw, Florence J. Hardy, Linus O. Johannissen, Thomas M. Lister, Emilie F. Gérard, Zachary Birch-Price, Richard Obexer, Sam Hay, Anthony P. Green*, A non-canonical nucleophile unlocks a new mechanistic pathway in a designed enzyme. Nature Communications, 15, 1956, (2024) Read here.

Elizabeth L. Bell, Amy E. Hutton, Ashleigh J. Burke, Adam O’Connell, Amber Barry, Elaine O’Reilly,* Anthony P. Green,* Strategies for designing biocatalysts with new functions. Chem. Soc. Rev, 53, 2851-2862, (2024) Read Here.

Adam O’Connell, Amber Barry, Ashleigh J. Burke, Amy E. Hutton, Elizabeth L. Bell, Anthony P. Green,* Elaine O’Reilly,* Biocatalysis: landmark discoveries and applications in chemical synthesis, Chem. Soc. Rev., 53, 2828-2850, (2024) Read here.

2023

Jingming Zhao, Ying Zhuo, Daniel E. Diaz, Muralidharan Shanmugam, Abbey J. Telfer, Peter J. Lindley, Daniel Kracher, Takahiro Hayashi, Lisa S. Seibt, Florence J. Hardy, Oliver Manners, Tobias M. Hedison, Katherine A. Hollywood, Reynard Spiess, Kathleen M. Cain, Sofia Diaz-Moreno, Nigel S. Scrutton, Morten Tovborg, Paul H. Walton*, Derren J. Heyes*, Anthony P. Green*, Mapping the Initial Stages of a Protective Pathway that Enhances Catalytic Turnover by a Lytic Polysaccharide Monooxygenase. J. Am. Chem. Soc., 145, 37, 20672–20682, (2023) Read here.

Zachary Birch-Price, Christopher J. Taylor, Mary Ortmayer, Anthony P. Green, Engineering Enzyme Activity Using an Expanded Amino Acid Alphabet. Protein Engineering, Design and Selection, 36, gzac013, (2023) Read here.

Emily Radley, John Davidson, Jake Foster, Richard Obexer, Elizabeth L. Bell*, Anthony P. Green*, Engineering Enzymes For Environmental Sustainability. Angew. Chem., 62, e20230930, (2023) Read here.

Euan J. Hossack, Florence J. Hardy*, Anthony P. Green*, Building Enzymes through Design and Evolution. ACS Catalysis, 13, 12436-12444, (2023). Read here.

Indrek Kalvet, Mary Ortmayer, Jingming Zhao, Rebecca Crawshaw, Nathan M. Ennist, Colin Levy, Anindya Roy*, Anthony P. Green*, David Baker* Design of Heme Enzymes with a Tunable Substrate Binding Pocket Adjacent to an Open Metal Coordination Site. J. Am. Chem. Soc., 145, 14307-14315, (2023) Read here.

Christopher J. Taylor, Florence J. Hardy, Ashleigh J. Burke, Riley M. Bednar, Ryan A. Mehl, Anthony P. Green*, Sarah L. Lovelock*, Engineering Mutually Orthogonal PylRS/tRNA Pairs for Dual Encoding of Functional Histidine Analogues. Protein Sci, 32, e4640, (2023) Read here.

Dr. Ashleigh J. Burke, Thomas M. Lister, Dr. James R. Marshall, Dr. Murray J. B. Brown, Dr. Richard Lloyd, Prof. Anthony P. Green*, Prof. Nicholas J. Turner*, Engineered Biocatalysts for Enantioselective Reductive Aminations of Cyclic Secondary Amines. ChemCatChem. e202300256, (2023) Read here.

Aoife Taylor, Shaowei Zhang, Linus O. Johannissen, Michiyo Sakuma, Robert S. Phillips, Anthony P. Green, Sam Hay, Derren J. Heyes, Nigel S. Scrutton, Mechanistic implications of the ternary complex structural models for the photoenzyme protochlorophyllide oxidoreductase. FEBS J., (2023). Read here.

2022

Jonathan S. Trimble, Rebecca Crawshaw, Florence J. Hardy, Colin W. Levy, Murray J.B. Brown, Douglas E. Fuerst, Derren J. Heyes, Richard Obexer, Anthony P. Green, A Designed Photoenzyme for Enantioselective [2+2]-Cycloadditions. Nature, 611, 709-714, (2022) Read here.

Elizabeth Bell, Ross Smithson, Siobhan Kilbride, Jake Foster, Florence J. Hardy, S. Ramachandran, A. Tedstone, S. Haigh, A. Garforth, P. Day, Colin Levy, Michael Shaver, and Anthony P. Green, Directed Evolution of an Efficient and Thermostable PET Depolymerase. Nature Catalysis, 5, 673-681, (2022) Read here.

Sarah L. Lovelock, Rebecca Crawshaw, Sophie Basler, Colin Levy, David Baker, Donald Hilvert, Anthony P. Green, The Road to Fully Programmable Protein Catalysis. Nature, 606, 49-58, (2022) Read here.

Rebecca Crawshaw, Amy E. Crossley, Linus Johannissen, Ashleigh J. Burke, Sam Hay , Colin Levy, David Baker , Sarah L. Lovelock  and Anthony P. Green, Engineering an efficient and enantioselective enzyme for the Morita–Baylis–Hillman reaction. Nature Chemistry , 14, 313-320, (2022) Read here. Highlighted in Chemistry World. Nature Catalysis research highlight. Nature Chemistry News & Views.

Ashleigh J. Burke, William R. Birmingham, Ying Zhuo, Thomas W. Thorpe, Bruna Zucoloto da Costa, Rebecca Crawshaw, Ian Rowles, James D. Finnigan, Carl Young, Gregory M. Holgate, Mark P. Muldowney, Simon J. Charnock, Sarah L. Lovelock*, Nicholas J. Turner*, Anthony P. Green*, An Engineered Cytidine Deaminase for Biocatalytic Production of a Key Intermediate of the Covid-19 Antiviral Molnupiravir. J. Am. Chem. Soc., 144, 3761-3765, (2022) Read here. Nature Research Highlight, ACS Editor’s choice, associated article in New Statesman, a prominent British political and cultural magazine ‘Democratising Medicine: Creating Fair Access to Medicines’ (in press)

2021

Elizabeth L. Bell, William Finnigan, Scott P. France, Anthony P. Green, Martin A. Hayes, Lorna J. Hepworth, Sarah L. Lovelock, Haruka Niikura, Sílvia Osuna, Elvira Romero, Katherine S. Ryan, Nicholas J. Turner and Sabine L. Flitsch, Biocatalysis. Nat Rev Methods Primers, 1, 46, (2021) Read here.

Mary Ortmayer, Florence J. Hardy, Matthew G. Quesne, Karl Fisher, Colin Levy, Derren J. Heyes, C. Richard A. Catlow, Sam P. de Visser, Stephen E. J. Rigby, Sam Hay, Anthony P. Green*, A non-canonical tryptophan analogue reveals an active site hydrogen bond controlling ferryl reactivity in a heme peroxidase. JACS Au.,1, 913, (2021) Read here.

Florence J. Hardy, Mary Ortmayer, Anthony P. Green, Claire E.M. Noble, J. L. Ross Anderson, Recent Advances in Understanding, Enhancing and Creating Heme Peroxidases, Reference Module in Chemistry, Molecular Sciences and Chemical Engineering, (2021) Read here.

2020

Mary Ortmayer, Karl Fisher, Jaswir Basran, Emmanuel M. Wolde-Michael, Derren J. Heyes, Colin Levy, Sarah L. Lovelock, J. L. Ross Anderson, Emma L. Raven, Sam Hay, Stephen E. J. Rigby, Anthony P. Green*, Rewiring the ‘push-pull’ catalytic machinery of a haem enzyme using an expanded genetic code. ACS Catalysis, 10, 2735, (2020) Read here.

Jingming Zhao, Ashleigh J. Burke, Anthony P. Green, Enzymes with Non-Canonical Amino Acids. Curr. Opin. Chem. Biol. 55, 136, (2020) Read here.

Calvin W Z Lee, M Qadri E Mubarak, Anthony P Green, Sam P de Visser, How Does Replacement of the Axial Histidine Ligand in Cytochrome c Peroxidase by Nδ-Methyl Histidine Affect Its Properties and Functions? A Computational Study. Int. J. Mol. Sci., 21, 7133, (2020) Read here.

Giulia Paggiola , Nolwenn Derrien , Jonathan D. Moseley , Anthony Green , Sabine L. Flitsch , James H. Clark , Con Robert McElroy and Andrew J. Hunt, Application of bio-based solvents for biocatalysed synthesis of amides with Pseudomonas stutzeri lipase (PSL). Pure Appl. Chem, 92 (4), 579-586, (2020) Read here.

2019

Ashleigh J. Burke, Sarah L. Lovelock, Amina Frese, Rebecca Crawshaw, Mary Ortmayer, Mark Dunstan, Colin Levy, Anthony P. Green, Design and Evolution of an Enzyme with a Non-Canonical Organocatalytic Mechanism. Nature, 570, 219, (2019) Read here. Highlighted in C&E News.

Aaron Debon, Moritz Pott, Richard Obexer, Anthony P. Green, Lukas Friedrich, Andrew D. Griffiths, Donald Hilvert, Ultrahigh-Throughput Screening Enables Efficient Single-Round Oxidase Remodeling. Nature Catalysis, 2, 740, (2019) Read here.

2018

Jin Xu, Anthony P. Green, Nicholas J. Turner, Chemoenzymatic Synthesis of Pyrazines and Pyrroles. Angew. Chem. Int. Ed. 57, 16760, (2018) Read here.

Takahiro Hayashi, Donald Hilvert, Anthony P. Green, Engineered Metalloenzymes with Non-Canonical Coordination Environments. Chem. Eur. J., 24, 11821, (2018) Read here.

Moritz Pott, Takahiro Hayashi, Takahiro Mori, Peer R. E. Mittl, Anthony P. Green*, Donald Hilvert*, A Non-Canonical Proximal Heme Ligand Affords an Efficient Peroxidase in a Globin Fold. J. Am. Chem. Soc., 140, 1535, (2018) Read here.

2017

Christopher J. Gray, Baptiste Schindler, Lukasz G. Migas, Martina Pičmanová, Abdul R. Allouche, Anthony P. Green, Santanu Mandal, Mohammed S. Motawia, Raquel Sánchez-Pérez, Nanna Bjarnholt, Birger L. Møller, Anouk M. Rijs, Perdita E. Barran*, Isabelle Compagnon*, Claire E. Eyers*, Sabine L. Flitsch, Bottom-Up Elucidation of Glycosidic Bond Stereochemistry. Anal. Chem. 89, 4540, (2017) Read here.

Anthony P. Green, Simon Hardy,  Eric J. Thomas, Synthetic Approaches to the C11–C27 Fragments of Bryostatins. Org. Biomol. Chem., 15, 9475, (2017) Read here.

Anthony P Green, Simon Hardy, Alan T L Lee, Eric J Thomas, Total Synthesis of 7-des-O-Pivaloyl-7-O-Benzylbryostatin 10. Org. Biomol. Chem., 15, 9497-9526, (2017) Read here.

Anthony P. Green, Elaine O'Reilly, Ortho-Xylylenediamine Dihydrochloride. In Encyclopedia of Reagents for Organic Synthesis, (2017) Read here.

2016

Anthony P. Green, Nicholas J. Turner, Biocatalytic Retrosynthesis: Redesigning Synthetic Routes to High-Value Chemicals, Perspectives in Science, 9, 42-48, (2016) Read here.

Anthony P. Green, Takahiro Hayashi, Peer R. E. Mittl, Donald Hilvert, A Chemically Programmed Proximal Ligand Enhances the Catalytic Properties of a Heme Enzyme. J. Am. Chem. Soc.,138, 11344, (2016) Read here. JACS Editor’s choice, Highlighted in ACS Chemical Biology

Andrew Gomm, William Lewis, Anthony P. Green, Elaine O'Reilly, A New Generation of Smart Amine Donors for Transaminase-Mediated Biotransformations. Chem. Eur. J., 22, 12692, (2016) Read here.

Anthony Green - Mentored Publications

A. P. Green, N. J. Turner, E. O’Reilly, Chiral Amine Synthesis Using ω-Transaminases: An Amine Donor Which Displaces Equilibria and Enables High-Throughput Screening. Angew. Chem. Int. Ed. 2014, 53, 10714. VIP article, Highlighted in C&E News 2014, vol. 92, Issue 35, p. 13., Highlighted in Nature Chemistry 2014, 6, 849, Highlighted in synfacts and selected for F1000Prime

A. P. Green, N. J. Turner, E. O’Reilly, Enzymatic Processes and Uses. Publication number WO2016009195 A1

J. W. Schmidberger, L. J. Hepworth, A. P. Green, S. L. Flitsch, Enzymatic Synthesis of Amides. Science of Synthesis, Biocatalysis in Organic Synthesis Volume 1, 329.

P. Both, A. P. Green, C. J. Gray, R. Šardzík, J. Voglmeir, C. Fontana, M. Austeri, M. Rejzek, D. Richardson, R. A. Field, G. Widmalm, S. L. Flitsch and C. E. Eyers, Discrimination of Epimeric Glycans and Glycopeptides using IM-MS and its Potential for Carbohydrate Sequencing. Nature Chem. 2014, 6, 65.

L. Heap, A. P. Green, D. Brown, B. van Dongen , N. J. Turner, Role of Laccase as an Enzymatic Pretreatment Method to Improve Lignocellulosic Saccharification. Catal. Sci. Technol. 2014, 4, 2251.

D. Ghislieri, A. P. Green, M. Pontini, S. C. Willies, I. Rowles, A. Frank, G. Grogan, N. J. Turner, Engineering an Enantioselective Amine Oxidase for the Synthesis of Pharmaceutical Building Blocks and Alkaloid Natural Products. J. Am. Chem. Soc. 2013, 135, 10863. Hot article, Highlighted in C&E News 2013, vol. 91, Issue 30, p. 34., JACS editor’s choice

D. Ghislieri, D. Houghton, A. P. Green, S. C. Willies, N. J. Turner, Monoamine Oxidase (MAO-N) Catalyzed Deracemization of Tetrahydro-β-carbolines: Substrate Dependent Switch in Enantioselectivity. ACS Catal. 2013, 3, 2869.

K. Adlington, A. P. Green, W. Wang, S. M. Howdle, D. J. Irvine, Direct ‘In Situ’, Low VOC, High Yielding, CO2 Expanded Phase Catalytic Chain Transfer Polymerisation: Towards Scale-up. Dalton Trans. 2013, 42, 127.

R. Šardzík, A. P. Green, N. Laurent, P. Both, C. Fontana, J. Voglmeir, M. J. Weissenborn, R. Haddoub, P. Grassi, S. M. Haslam, G. Widmalm, S. L. Flitsch, Chemoenzymatic Synthesis of O-Mannosylpeptides in Solution and on Solid Phase. J. Am. Chem. Soc. 2012, 134, 4521.

P. Both, R. Sardzik, M. Weissenborn, A. P. Green, J. Voglmeir, S. L. Flitsch, Glycoarrays on Gold Surfaces - Cracking the Sugar Code by Navigating the Glycospace Proceedings of the 2nd Beilstein Symposium on Glyco-Bioinformatics, 2012, 93.

A. P. Green, A. T. L. Lee, E. J. Thomas, Total Synthesis of a 20-Deoxybryostatin. Chem. Commun. 2011, 47, 7200. Hot article

A. P. Green, S. Hardy, A. T. L.  Lee, E. J. Thomas, Approaches to the Total Synthesis of Biologically Active Natural Products: Studies Directed Towards Bryostatins. Phytochem. Rev. 2010, 9, 501-513.

J. V. Allen, A. P. Green, S. Hardy, N. M. Heron, A. T. L. Lee, E. J. Thomas On the use of the Modified Julia Olefination for Bryostatin Synthesis. Tetrahedron Lett. 2008, 49, 6352-6355.

A. P. Green, S. Hardy. E. J. Thomas, A Synthesis of the C(17)-C(27) Fragment of Bryostatin 1. Synlett 2008, 14, 2103-2106.